Glucose and phosphate identification HPLC

[Dexter1]

Hello ,


I have tried to use different columns such as Aminex HPX-87H Column 300 x7.8 mm, Agilent Hi-PLex H, 7.7 x 300 mm, NUCLEOGEL SUGAR 810 H, 300x 7.8 mm... but none of these manage to separate the two peaks that remain overlapping... does anyone perhaps know of a different column to use or suggestions for parameters considering the analytes? Or any suggestions considering the properties of the compounds ?

Best,

[JMB]

Have you considered the well-known ability of sugars to form complexes/compounds with boric and boronic acids??

See,

"Interactions between boric acid derivatives and saccharides in aqueous media: Structures and stabilities of resulting esters", Joop A. Peters, Coordination Chemistry Reviews, Vol. 108 (2014), 1-22

https://doi.org/10.1016/j.ccr.2014.01.016

Note that phosphate ALSO reacts.....

Please give us an update if you are/are not successful with this methodology.

JMB

[Dexter1]

Hi JMB, yes, I am also considering the derivatisation of glucose with ABEE or PMP... thank you for your suggestion, the only concern is that the phospahte also reacts, so we should test how it affects the RT for both glucose and phospate... I am doing a bit of research if I test boric acid I will contact you