Stability indicating HPLC method for Minocycline

[Raphy]

Can anyone please refer me to a stability indicating HPLC method for testing minocycline and its degradation products? Any publication?
Or any advice?
Thanks!

[mbicking]

Use an acidic mobile phase (0.1% formic acid or 0.1% phosphoric acid) with methanol. Detection at about 260 or 360 nm.

A standard reversed phase column may work, but I had better separation with a Zorbax SB-AQ column. Try about 50% methanol. I was able to see the main peak and two main impurities.

[yaoguocan]

the hplc method for determiation of it has been published in one of chinese journal . the abstract of the method as follows:
mobile phase:water（20mmol/l ammonium acetate pH=6.5）:ACN:MeOH=830:185:5
colum type:C18
wavelength:280nm

[SIELC_Tech]

Here is method used by one of our customers for Tetracycline stability method:
http://www.sielc.com/compound_285.html
Your compound is from the same class of compounds and can be analyzed in a similar fshion.

Regards,

Vlad

[laserman]

This is excerpt from Waters application note book.
http://www.waters.com/webassets/cms/lib ... try168.pdf

Best regards,