how to quantify Carbomer 980 on HPLC ?

[dhruvil81]

Hi all
This is dhruvil working with a pharmaceutical company as Research Officer in an Analytical Development Dept.(Formulation).

Can anyone suggest me the method for quantifying Carbomer 980 by using HPLC technique ?

Please suggest me if anyone know bout this as early as possible.

Thanking you all

Regards

Dhruvil

[juddc]

I don't think carbomer would necessarily be amenable to HPLC analysis. I think it's just too big to elute from any HPLC or SEC column.

You might try to isolate it (dilute acid and polar organic solvent would be a start) then do some spectroscopy or titrate the carboxylic acid groups and calculate it that way. Quantification of carbomer in a product is certainly an interesting question, but I don't think that HPLC is the answer here.