Peakbroadening of Peptide

[HW Mueller]

It seems there got to be a way to get rid of the TFA, even if you have to use some more solvent. If you use a solvent with less elution power than mobile phase you can inject much more volume. Uwe worked out something like that?

[HW Mueller]

It seems there got to be a way to get rid of the TFA, even if you have to use some more solvent. If you use a solvent with less elution power than mobile phase you can inject much more volume. Uwe worked out something like that?

[Alex]

Great! I doubled the buffercontent from 0.5 % AcOH to 1 % AcOH and the peakshape is much better.
Additionally I swiched to the Nucleodur RP-C18 CN column (pyramid) wich helped also.
Now I will try to raise the flow in order to "squeeze" the peak together.

This guides me to another question: Is there a reasonable border to which raising the buffercontent doesnt make sense anymore? Because I never red of buffervalues over 1 %.

Thank all of you for your help!!!!!!!

[kiknos]

Great! I doubled the buffercontent from 0.5 % AcOH to 1 % AcOH and the peakshape is much better.
Additionally I swiched to the Nucleodur RP-C18 CN column (pyramid) wich helped also.

excuse me, what is RP-C18 CN column? mixed C18 and CN as stationary phase? i am curious.

[Alex]

It is a hydrophilic endcapping which enables a mobile phase with 100 % Water without collapsing of the C-18 chains. See also here:

https://www.macherey-nagel.de/web/MN-WE ... NDURC18PYR

Regards,
Alex

[Steve]

There is a great deal on the Pyramid and Gravity Columns offered By Macherey-Nagel. If you check the Announcement Board of this forum Macherey-Nagel is offering a Buy One get One Column free until 02/15/2005.

[kiknos]

It is a hydrophilic endcapping which enables a mobile phase with 100 % Water without collapsing of the C-18 chains. See also here:

https://www.macherey-nagel.de/web/MN-WE ... NDURC18PYR

Regards,
Alex

Alex and Steve, thanks for the kind information!

one problem for me is everytime i tried to load M-N web site, i always got safety warrenty. so i had to give up as i know so little about computer and net and i don't want to get any virus or safety risk.

[MG]

I get that warning too. Something about the name on the Verisign certificate not matching the site name. I think this would only be a security issue if you were going to order stuff through the site using a credit card number etc.