Role of THF in RP-HPLC separation

[praveenpaliwal]

Hellow ,

I am praveen and I want to know that waht is the role of THF in RP-HPLC separation. Either the selection of THF is compound basis or It has some property for achiving separation.

Thanks and regards

praveen

[Uwe Neue]

The most important role of THF is the changes in selectivity that you get with the addition of this solvent. They are larger than what you get from a comparison between acetonitrile and methanol.

Contact me and I send you a publication!

[praveenpaliwal]

Sir,

I want to know more about Role of THF , as you said about publication.
Please send me .

Thanks and regards

praveen

[Austin]

Dear Uwe Neue:

I'll appreciate you very much if you send me a publication as you said, and I hope it help me.

Last week I performed an analysis, and HPLC conditions as follows:

Column: Waters Atlantis, 4.6x100mm, 3 micron, C18
Mobile phase: water-acetonitrile-THF(2:1:1)
Detection: UV 244nm
Column Temp.:25oC
Flow rate:1.0ml/min

When I tried consecutive check standard injections, an unknown peak existed and its retention time increased slowly, which interfered the interest content. Our director said some error existed in THF. Then I selected a new batch, OK, the "stranger" peak existed no longer. What happend in THF.

[Austin]

Dear Uwe Neue:

Thank you for your help!

I've read your publication and learned the role of THF, but I didn't get the answer of the problem above and I think whether the peroxides in THF lead the unknown peak's retention and interfere with the interest peak's content, I read that THF may be contain some peroxides under improper storage condition on books and the old batch was placed improperly(near to muffle furnace and exposured to light, and opened many times) in our lab, OK, what's the organisms and please give me some ideas

Thanks and best regards

Austin

[Uwe Neue]

A good way to prevent (or at least minimize) the formation of peroxides in THF is to keep the solvent in brown bottles and to sparge with nitrogen.

[bhuvfe]

Dear Uwe Neue,

I'd like to see that publication as well.
Thank you in advance.

[Uwe Neue]

U. D. Neue, A. Méndez, “Selectivity in Reversed-Phase Separations: General Influence of Solvent Type and Mobile Phase pHâ€

[bhuvfe]

Dear Uwe,

Please send the publications at this email address :bhuvfe@yahoo.com.
Many thanks.

[aspoul]

Dear Uwe,

Would you please send the publications at this email address :aspoul@163.com

Thanks a lot .

[moonchips]

Dear Uwe,
Could you please send me the publication too?

liziman@yahoo.com

Many thanks

[MineSweeper]

Dear Uwe,
I've searched the publications in PubMed. Unfortunately, there is only the abstract of the first publication. Would you please also send me the copy for these publications?

Thanks a lot.
my E-mail adress:
ht_hh@web.de

[moonchips]

Dear Uwe, I have got the articles.

Thanks a lot!
ziman

[JoeFish]

Uwe Neue,

If you have the time.

Could you send me these articles, I am most interested.

joseph.holcombe@alconlabs.com