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- Posts: 12
- Joined: Mon Oct 29, 2007 3:51 am
dear collegues
i m looking for a reverse phase chromatographic system to separate hidrocortisone and prednisolone (resolution solution).
I tried with a L3 column and i used water and THF (70:30) in my mobile phase.
I used a L1 column too. Methanol and CH3CN dont work.
The molecules both,show the same retention time...
I use a UV detection at 254 nm-
Many thanks for your comments
Best Regards
Paul
i m looking for a reverse phase chromatographic system to separate hidrocortisone and prednisolone (resolution solution).
I tried with a L3 column and i used water and THF (70:30) in my mobile phase.
I used a L1 column too. Methanol and CH3CN dont work.
The molecules both,show the same retention time...
I use a UV detection at 254 nm-
Many thanks for your comments
Best Regards
Paul
