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Setting up a sequence in Agilent ChemStation

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I'm a complete newbie to LC, so my question is really basic (and I could not figure it out after reading the manual!)
I'm trying to set up a sequence to run overnight on our Agilent. Let's say I have 10 samples in 10 vials, and I want to execute the same method for each sample.
I have all of this in my Sequence Table Editor, however, when I start the sequence, it only injects sample 1 (i.e. Vial 1), runs the method, then stops. It doesn't move on to the next samples.
What am I doing wrong?

Hi

What you need to do is toggle between Method task and sequence task.
These buttons are found at the top left of the main on line screen.Method task button is a picture of one vial ,sequence task button is a picture of 3 vials. The sequence task button must be selected to run a sequence .

Hope this helps . If you need any further help (chemstore, reports,calculations, calibration quantification etc) on this software please post query.In my opinion the software is very dated but it is fairly straightforward to pick up.

Thanks
Andy

Hi Andy,
I do have the Method Task button selected. However, the sequence still stops after executing the first step.

As Abell stated, you are executing "Run Method" instead of "Run Sequence". Go to second column Run Control, third choice down in Run Sequence.

Thanks for the help, guys, I have figured out what the problem was. (I called Agilent tech support!)
The reason that the sequence was only running the first method, and then stopping, was because in my method, I did not specify a pump stop time. So basically, the sequence would run the method, and since the method never ended, it couldn't move on to the next step/method.
5 posts Page 1 of 1

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