Risedronate help

[fferron]

I need to analyse Risedronate. I would like to know if someone have information about the use of HILIC columns and could give me some bibliographic references.
Many thanks
Francisco

[SIELC_Tech]

Here is article on analysis or alendronic acid which is analog of residronic acid. One of our customers validated method using Primesep SB mixed-mode HPLC column.
"Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector" by Liu, X.K., Fang, J.B., Cauchon, N., Zhou, P., published in Journal of Pharmaceutical and Biomedical Analysis, Vol. 46, No. 4, pages 639-644 (2008)

Regards,

Vlad

[SIELC_Tech]

Here is article on analysis or alendronic acid using Primesep SB mixed-mode HPLC column.

"Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector" by Liu, X.K., Fang, J.B., Cauchon, N., Zhou, P., published in Journal of Pharmaceutical and Biomedical Analysis, Vol. 46, No. 4, pages 639-644 (2008)

These are LC/MS compatible conditions. Proteins from plasma will repel from stationary phase as both stationary phase and plasma components will be positively charged.

Here is the link for methods developed in our lab:

http://www.sielc.com/compound_248.html


Regards,

Vlad

[Uwe Neue]

At the Waters website, you can get a complete method for bisphosphonate in plasma or urine, including a HILIC method. Search under alendronate or for the application sheet 720002454EN!

[Carl Sanchez]

bisphosphonates are very strong chelators often necessitating the use of additives and/or derivatization. Do you need an MS compatible method?