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amino column : reverse or normal ?

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Hello,
I want to separate sugars with an amino column and a RI detector. The mobile phase should be CH3OH/H2O (75/25). As the mobile and the stationnary phase are polar, is it a normal or a reverse phase chromatography ?
Thank you

If you increase the amount of ACN in the mobile phase, retention increases, therefore it is, technically, normal-phase. If this technology were developed today, it would be classed as HILIC (see other threads on that topic).
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts Page 1 of 1

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