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Weird Tailing?

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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syx
Can you give us the column dimensions, flow rate, and the details how you bring the solvent to temperature?
Column dimension: 30 x 2.00 mm, with 2.5 um particles.
Flow rate: 1.0 mL/minute.
Column oven: forced air-circulation-type (without pre-heating)

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Uwe Neue
My suggestion is to try preheating. It may sharpen up your peak, since temperature is an important factor in the speed of this equilibrium.

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syx
Thanks, Dr. Neue. Unfortunately, we do not have column oven with pre-heating system right now.
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