Advertisement

Autosampler temperature

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Hi I have a method with a sample temperature 4 degrees +- 1 degree. I will be doing that method on waters alliance sistem and waters arc sistem. Can this alarm band +-1 degree be a problem.

Thank you
Yes.

4°C is as low as the sample compartments will go under good conditions and ±1°C is pretty tight.

Anticipate alarms. That said, I think you can tell the Alliance to ignore alarms via Empower...

If having sample temp. controlled that tightly is not really required, loosen it up a little and make your life easier.
Thanks,
DR
Image
Yes I think that alliance sistem only shows warnings but doesn’t stop run sample but I don’t know about waters ARC sistem if it stops injecting sample when temperature is over alarm band
3 posts Page 1 of 1

Who is online

In total there are 22 users online :: 1 registered, 0 hidden and 21 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Baidu [Spider] and 21 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry