Gradient pump calibration calculation

[AHK]

Hi everyone,

Have you heard of a software call Gradient pump calibration calculation (GPCC), if so where can I get it from. This sw is to calculate the gradient performance of my hplc

Thanks

[DR]

Step test and gradient formation linearity tests are commonly done to check this. Nothing more than water, acetone (0.3% in water), and (maybe) Excel are required.

[dvdthomas345]

Can anyone let me know the calculation needed to check the Gradient of HPLC using 0.1% Acetone in water?

[tom jupille]

https://www.lcresources.com/tsbible/22102004.pdf