96-Well Plates

[Stryder08]

I am running a Waters Acquity UPLC with an LCT Premier XE (TOF). I have tried to use well plates in the past, but have always picked up contaminants from the cover films we have used. I really don't want to use aluminum foil, but if I have to, then I will.

Anyone ever use well plates? If so, what type of cover film do you use? We cannot use any adhesive-based film as the adhesive builds up on the needle. I have also tried using a pressue type seal (which is preferable), but had some contaminants due to the high sensitivity of the MS.

Suggestions?

[matthew]

Agilent makes 96 well plates, with glass inserts and septa:

http://www.chem.agilent.com/ecommerce/p ... =5065-4402

Aluminum foil will work fine if you aren't storing the plates for a long period of time.

[JJG]

I have used the plate mats from Sun Sri quite a bit, though they aren't exactly cheap. Have worked well for me.

http://www.sun-sri.com/life_science/micromat.aspx

[Roxanne42]

We use Symyx plates, where the glass vials are covered with a teflon sheet, followed by one or two black rubber sheets (to seal) and then a metallic plate - all screwed down.

The teflon sheet is the only thing we have seen that we ensure that reactions don't get contaminated (lots of organic solvents are used!) - but we do run into the problem that once the sheet is pierced, some evaporation can occur through the aperture (especially when heating) and this can sometimes be a problem in analysis.

Haven't tried the SunSRI micromats yet (teflon-coated silicone and apparently resealable) so let me know if you do. They aren't cheap though (and neither are Agilent's little full plates!) - at least definitely not from an educational facility standpoint|!

[brewer]

Microliter also provide an option:

http://64.25.2.94/cgi-bin/nlnetupd?LINK ... eqr_type=O