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By mary on Saturday, July 24, 2004 - 11:18 am:
hi,
what do you think about using solvents that aren´t HPLC grade?
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By Anon on Sunday, July 25, 2004 - 08:48 am:
As long as the solvents carry a specification that certifies low ionic and organic contaminants. HPLC grade simply ensures this. If you have an HPLC grade solvent in the lab compare this specification with the grade that you plan to use. I am assuming you plan to use this for HPLC.
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By FRED on Tuesday, July 27, 2004 - 07:06 am:
Cheap solvents may not have the HPLC grade certificate, and you may test a bottle and it may perform well enough for your application, however, bottles quality will varry over time from the same supplier. So you put your analysis at risk. What is the cost to you if the analysis / result is incorrect. Usually the answer is sufficient enough for you to warrant the purchase of pucker HPLC analytical grade reagents. Though I have even been caught out with so called HPLC grade reagents being out of spec. This is why for each run / batch it is important that blanks / standards / internal standards are used to ensure full recovery and that the whole HPLC system is working correctly within previously tested limits.
Common sense really.
hi,
what do you think about using solvents that aren´t HPLC grade?
-------------------------------------------------------------------------------------------------------
By Anon on Sunday, July 25, 2004 - 08:48 am:
As long as the solvents carry a specification that certifies low ionic and organic contaminants. HPLC grade simply ensures this. If you have an HPLC grade solvent in the lab compare this specification with the grade that you plan to use. I am assuming you plan to use this for HPLC.
-------------------------------------------------------------------------------------------------------
By FRED on Tuesday, July 27, 2004 - 07:06 am:
Cheap solvents may not have the HPLC grade certificate, and you may test a bottle and it may perform well enough for your application, however, bottles quality will varry over time from the same supplier. So you put your analysis at risk. What is the cost to you if the analysis / result is incorrect. Usually the answer is sufficient enough for you to warrant the purchase of pucker HPLC analytical grade reagents. Though I have even been caught out with so called HPLC grade reagents being out of spec. This is why for each run / batch it is important that blanks / standards / internal standards are used to ensure full recovery and that the whole HPLC system is working correctly within previously tested limits.
Common sense really.
