Test Mix for HILIC

[Hollow]

Hello

I'm looking for a simple and convenient test mix for HILIC (Atlantis) columns for the purpose of performance check of the column over its lifetime.
(k' and N)

It should contain of a t0 marker and 2-3 substances which can isocratically separated.

Does anybody have something just by hand?

Thank you

Edit:
I'v googled a bit and found something at Alltech
Toluen, Hydantoin, Uracil, Caffein, Allantoin
ACN/NH4COOH pH3 95/5

sounds convenient to me and I have most of the substances in our lab.
I will give it a try.

As I've searched on the forum, toluen is a good choice for t0 marker isn't it? Or is naphthalen better?

[Patrik Appelblad]

We use Toluen (for t'0), uracil and cytosine.
Naphtalene is an equal good choice for t'0 but it smells....

At 80% acetonitrile, low ionic strength of buffer (5 mM overall) and neutral pH Uracil and cytosine elutes with retention factors between 0.5 and 2.0 on our ZIC®-HILIC phases, depending on pore and particle size.

These compounds are sufficiently stable (several months in solution in refridgerator and weeks at RT) and will be a simple and convenient test mix for column performance check (k' and N). They can also be used with different detection modes.

[Hollow]

Thank you Patrik for your reply.

After some trials I used the following mix:

Toluene 1 µl/ml
Caffein 60µg/ml
Theobromine 60µg/ml
Adenosine 110 µg/ml
dissolved in H2O/MeOH/ACN 10/18/72

HPLC conditions
isocratic, premixed: 5 ml NH4COOH 200mM, pH 3.0 / 95 ml ACN
T Col: 30°C
Det: UV 270 nm
Inj Vol: 5 µl

With this I get the following retention factors on Waters Atlantis Hilic, 3µm 4.6x100
Toluene := 0
Caffein 0.6
Theobromine 0.9
Adenosine 3.3