Total Reducible Sugar Analysis in Cane Molasses by GC orHPLC

[ATUL MITTAL]

Hi Everybody,
i want to analyse total reducible sugar in cane molasses by GC. Please provide me analysis method if anyone is doing this type of analysis.

Thanks in advance to you all.

[k.m.seenivasan]

hi
i am doing sugar analysis by HPLC.Are you interested in HPLC?Reply

[ATUL MITTAL]

Thanks for your reply, Please send me the complete method of HPLC so that i can be able to analyse sugars by HPLC. You can send me the method by my e mail also. My e mail ID is amittal@indiaglycols.net.

Waiting for your reply.

[leadazide]

Please also post a short describtion of the methode here please..

[k.m.seenivasan]

Determination of sugars by HPLC.

Detector :Refractive Index Detector
Column :Ion Exchange column( i used Aminex HPX-87P FROM BIORAD)
cOLUMN TEMPERATURE : 80 C
Mobile Phase : Water
Flow : 0.6 ml/min

The column separated Dextrose,fructose and Sucrose.Maltose lies between dextrose and sucrose which we can not separate.So i have selected dextrose,fructose and sucrose.

The instrument used is Agilent 1100 HPLC.
I have tried this in C18 Column but it didn't works.

Kind Regards

Seeni

[k.m.seenivasan]

If you need the method of extraction of sugars in foods which contains fat and protein?Mail me.I'll send.