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Choosing of X and Y axis in linearity plot

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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During linearity plot why we are taking concentration in Xaxis and response(area) in Yaxis. why not the opposite?

Because x is always the independent quantity and y - the dependent quantity.
The response of the analyte (y quantity, e.g absorbance units) is always dependent on the amount (independent quantity) of the analyte (e.g its concentration).

Regards

For the same reason that maps are drawn with North at the top: doing it one consistent way makes things easier to interpret. In principle, the independent variable could just as well be shown on the vertical axis, but that would confuse people.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374

It is a convention but it is important to stick to it if you are calculating the regression mathematically.

The calculation of the line of regression assumes that the x values are known precisely and all the variation is in the y values. (eg peak area or height in HPLC)

thanks dear all
5 posts Page 1 of 1

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