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retention time stability

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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zokitano
Rick,

Does your colleague use helium to sparge the m.f. solvents while the HPLC instrument is operating?

best wishes

avatar
rick1112
hi
no he doesnt....
its a smiple RP method...using 100% ACn +0.1% TFA and 100% water+0.1% TFA(gradient run)..C18 column...
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