Hi Tim
The source of high backpressure can be due to
A) Higher than recommended backpressure to the cell. The recommended backpressure is 40 psi.
B) High backpressure from the cell.
C) High backpressure from the connecting tubings or the waste line.
Typically A) is the common cause of the leakage problem.
To test the contribution from the backpressure coil do the following:
1) Disconnect the lines in and out of the eluent ports of the leaking suppressor and connect them togather using a coupler.
2) Disconnect the lines in and out of the regen ports of the suppressor. Ensure that the line from the regen out port to the waste is a clear 1/8" tubing. This line can cause backpressure if the ID is < 0.02" and can cause suppressor leakage.
3) Turn on the pump at the application flow rate and measure the total system pressure. Let us call this X psi.
4) With the pump running disconnect the line out of the cell out port on the cell.
5) Measure the system pressure. Let us call this Y psi.
6) X-Y should be less than 40 psi. IF this is not the case then replace or trim this tubing to achieve 40 psi.
Now to test the contribution from the cell,
7) Disconnect the line (from the suppressor) to the cell in port.
8) Measure the pressure. Let us call this Z psi.
9) Y-Z should be less than 100 psi at 2 ml/min or less than 50 psi at 1 ml/min.
10) If the pressure of the cell exceeds the cited numbers in step 9 then replace the cell.
Note the contributions from any connecting tubings. Occassionally when the connections are overtightened this can cause high backpressure. Ensure that the tubing connections are all finger tight with a quarter turn with the wrench.
For more info the CSRS manual should contain all the trouble shooting information. This is available at Dionex.com at the following link.
http://www1.dionex.com/en-us/webdocs/ma ... RA_V14.pdf
