pH effect???

[rick1112]

hi
does pH of the sample injected matters(or affect) the stationary phase stability???

[tom jupille]

No single answer to that. If the mobile phase is well-buffered and the sample is small and/or dilute, there should be no problems. If your samples are large / high ionic strength / extreme pH, then a guard cartridge would be a good idea.

[rick1112]

hi
i have samples ranging from pH 3 to pH 9, the injection volume for these samples will be 100 microlitmy buffer is ACN+% TFA and water +0.1%TFA??
under these condition can the pH effect stationary phase

[Mark Tracy]

A guard is probably a good idea for this case, unless the stationary phase is rated for pH 9.

[HW Mueller]

My guess would be that your biggest problem could be pH incompatibility with resulting bad peak shapes (at the higher sample pH):