Advertisement

split & splitless

Discussions about GC and other "gas phase" separation techniques.

4 posts Page 1 of 1
please give me details about split mode
1:100 means howmuch sample analysed
why we are useing RI detecter as a universal detecter,every compound in universal different RI .
why not find out all compounds by UV detecter.

It means that 1 part of 100 parts is analysed, the rest (99 parts) is put out

A word of caution: The split ratio is only a relative measure of how much sample is entering the column as opposed to going out the split vent. For example, do not assume that a split of 10:1 is delivering exactly 10 times as much sample as a split of 100:1.

In my experience a properly functioning injection port will deliver 10 times as much compound on column at a 10:1 split compared to 100:1.
4 posts Page 1 of 1

Who is online

In total there are 7 users online :: 2 registered, 0 hidden and 5 guests (based on users active over the past 5 minutes)
Most users ever online was 5108 on Wed Nov 05, 2025 8:51 pm

Users browsing this forum: Majestic-12 [Bot], Semrush [Bot] and 5 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry