method for avobenzone, octinoxate and OS

[bumblebee_allison]

hi,
i'm new to this.

Does anyone have a method for separating Avobenzone, MCX and OS? i'm using a phenomenex column.
thanks,

[Bruce Hamilton]

I suspect you don't know that Phenomenex sells at least several hundred different HPLC columns, so please share with us at least the Catalogue number, if not the description on the label.

You also might like to tell us a little more about the HPLCs you have access to. ( eg what sort of pumps, detectors, columns etc. ).

If feeling really generous, you could decribe your sample and what work you have already perform to try and achieve the separation.

Bruce Hamilton

[Mark Tracy]

We did those a while back. A good C18 column, MeOH:H2O:HOAc 85:15:0.5, UV 310. A PDA is nice if you have it. The fun is in the extraction. We used Accelerated Solvent Extraction. I can send a note if you want.

[Consumer Products Guy]

J. DiNunzio and R. Gadde, Journal of Chromatography 519 (1990), pp. 117-124 is a good resource for sunscreen determinations by HPLC. Also see http://www.hamiltoncompany.com/HPLC/hxsil.asp

Bumble - you might try writing the complete names of your analytes of interest to get better responses in the future. I'm guessing you mean octyl salicylate for OS and mybe octyl methoxycinnamate (or 2-ethylhexyl methoxycinnamate) for MCX, but it's just guessing.

[bumblebee_allison]

thanks very much.

I was having problems separting the compounds MCX and avobenzone. I used the synergy column C18 RP at 90/10 MeOH/H20(0.1%TFA) at 310nm. I'm using a Waters with a UV detector.

[Bruce Hamilton]

As well as the excellent suggestions above, you may obtain useful information from;-

THE CHEMISTRY AND HPLC ANALYSIS OF CHEMICAL SUNSCREEN FILTERS IN SUNSCREENS AND COSMETICS
K. Lee Granger and P. R. Brown
Journal of Liquid Chromatography & Related Technologies
Volume 24, Number 19 / 2001, Pages 2895 - 2924

Analytical methods for the determination of persistent ingredients of personal care products in environmental matrices
Aaron M. Peck
Analytical and Bioanalytical Chemistry
Issue Volume 386, Number 4 / October, 2006, Pages 907-939

Bruce Hamilton

[Uwe Neue]

Two possibilities:

1. Try the separation in 70% acetonitrile on the column that you have, and if it does not work from the start, play with the ratio of acetonitrile and water.

2. Use an XBridge Phenyl column, with about 80% water, but keep the rest of the mobile phase the same.