NIR Spectroscopy

[Liza]

Hello all:


I am lost with quantitative NIR spectroscopy -


(1) Could someone explain the calibration process leading to "true vs. predicted" graph?
(2) How to validate the proposed method?


Please help.


Thanks.


Liza

[bartjoosen]

I can answer on your first question: its a graph where the actual amounts, used for calibration, are graphed against the expected amounts from the calibration line. This line should be lineair through zero, and have an R2 of 1, in a perfect world.

How to validate? I don't know, if it's just a validation like any other assay, you should demonstrate precision, accuracy, linearity,... aren't there any guidelines available?