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- Posts: 9
- Joined: Tue Aug 31, 2004 4:11 am
Hi,
I am currently using cleavable ICAT for protien quantation of a pathogen grown in 2 different conditions. I'm facing a huge problem in the labeling/identification of ICAT pairs and have been working on this for months........ The test using laminin and BSA works well for me, but not the actual runs. I'm not sure now if it is my methods (I've followed the exact method supplied by ABI and also tried that of Gygi et al) or the mass spec as there are always very very few proteins identified.
Could anyone advice me on their sucess rate on complex proteome and if any variations they have made on the protocols?
Thank you and best regards to all.
Ching Seng
I am currently using cleavable ICAT for protien quantation of a pathogen grown in 2 different conditions. I'm facing a huge problem in the labeling/identification of ICAT pairs and have been working on this for months........ The test using laminin and BSA works well for me, but not the actual runs. I'm not sure now if it is my methods (I've followed the exact method supplied by ABI and also tried that of Gygi et al) or the mass spec as there are always very very few proteins identified.
Could anyone advice me on their sucess rate on complex proteome and if any variations they have made on the protocols?
Thank you and best regards to all.
Ching Seng
