pressure problem with HPLC

[kupikupi]

hi.. i need help..
while i was injecting the standards, the peaks that came out were very messy.. when i checked the pressure, it was very low.. i tried to restart everything but nothing worked.. the pressure once increased but it decreased again..

anything wrong with my HPLC? anyone can help me? it's very urgent
thx..

[n-cute]

hi,
you should make some diagnostics I think. e.g. leak test ("How to" should be in your manual). By the way use water as mobile phase and check wherever you can all the way it goes for leakage. thats all, what I can tell You know, I'm quite newbie.

[danko]

Hi kupikupi

Judging from the information you share with us, I’d say you have air in one or both pumps.
Prime your pump/s and suck a good portion of mobile phase using a syringe. Prospectively it might be a good idea to degas your mobile phase/s prior to use.

Best Regards

[Jamie]

Kupikupi:

Happened to me several times and most of the time I run out of mobile phase and the pump sucked air.

Jaime

[rolpe]

Hi,

check following parameters:

How is your pressure, higher or lower than normal ?

If lower check for leaks. Tighten the fittings and check if purgevalve is properly closed.

Are there pressure changes ?
If there are high pressure changes >30 bar clean your check valves, especially if running ACN or buffers
If pressure difference between two pumps is about 10 bars change theinline filter.

Hope I can help you with this, otherwise download a troubleshooting guide (supelco etc). They are sometimes pretty helpful.

Best Regards

Rolpe

[unicorn]

hi,
you should make some diagnostics I think. e.g. leak test ("How to" should be in your manual). By the way use water as mobile phase and check wherever you can all the way it goes for leakage. thats all, what I can tell You know, I'm quite newbie.

I agree with you, if system has bubbles the pressure will be change like a sine function corresponding to the pulsations of the pump.
It is better to check leakage at joints, valve.

[diana]

Yes, I would also check the valves and carry out a pressure test.


Good luck,
Diana