ZIC-HILIC Sugars (monosaccharides)

[Aylin]

Hi! I'm looking for someone who has experiences in the separation of Sugars. I'm working with a LC-MS (UPLC) with the "SeQuant® ZIC®-HILIC 3.5µm,100Å 100 x 2.1 mm" column from Merck. I try to separate fructose, glucose, sorbose, psicose and sucrose. But unfortunately it's quite hard to get good results.

Anybody out there with practical knowledge?

Thanks!

Aylin

[AnaChem1983]

What appear to be the main problems with the analysis?

I've conducted analysis of sugars successfully in the past using NH2 based columns under reverse phase conditions with a MeCN:Water mobile phase (75:25) and refractive index detection. This was for the separation of sugars in foods and beverages.

[Andy Alpert]

First of all, any analysis that involves 75% ACN is using HILIC conditions, not reversed-phase. Second, different types of HILIC columns differ substantially in their selectivity. A "zwitterionic" coating will behave differently than will a neutral coating (diol; amide; etc.) or an amino (NH2) coating. I recommend you look up papers on the use of HILIC to separate the particular sugars you're interested in and then use the column that they used.

Does Merck-SeQuant claim that the column you have can separate the particular sugars you've listed?

[AnaChem1983]

First of all, any analysis that involves 75% ACN is using HILIC conditions, not reversed-phase

I stand corrected