Single Point calibration Hydrocarbon Analysis

[seamoro]

We are setting up two methods of analysis for hydrocarbons (gas and liquid methods) for the first time on new instruments in a new lab. I believe a single point is insufficient and inaccurate for determination. Single point calibration is only suitable when concentration is very close to the actual sample therefore there is less potential for error. I believe we have a couple of options
1. Low-Medium-High concentration's of standard which will give us an accurate cali curve
2. three points with a concentration very close to the expected concentration of the sample.
Basically im looking for the most accurate way of quantifying with the least possible error

Just wondering what are your thoughts on this?

[alphananotech]

Single point with concentration close to sample can work well if you use internal standard with samples and standards and calculating concentration by area ratio. To proof method precision, LOD/LOQ 6 replicates of the standard can be run, area RSD should be within 5%.
For polar carbohydrates we use LC/MS.