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- Posts: 25
- Joined: Tue Sep 17, 2013 1:03 pm
Hi,
we have a new LCQTOFMS system. I have run 18 standards individually and i was able detect all of them. the retention times was very close for some of the compounds (at a difference of 0.001 min for a few). Then i prepared a mix of them at same concentration and i was able to detect only 7 compounds. i am using agilent mass hunter qualiatative analysis software to detect the molecular features. is there some kind of co-suppression happening due to too close elution or is there something to tweak in the software or the gradient needs to be extended. Can somebody provide some inputs. Thanks.
we have a new LCQTOFMS system. I have run 18 standards individually and i was able detect all of them. the retention times was very close for some of the compounds (at a difference of 0.001 min for a few). Then i prepared a mix of them at same concentration and i was able to detect only 7 compounds. i am using agilent mass hunter qualiatative analysis software to detect the molecular features. is there some kind of co-suppression happening due to too close elution or is there something to tweak in the software or the gradient needs to be extended. Can somebody provide some inputs. Thanks.
