peaks vs retention time

[muggle]

Hi,

Is it possible to conclude that substance is present in the sample even if the peaks are not well-define and there is overlapping present in the chromatograph but the retention time is approximately the same.

If not, how to i improve the peaks if according to literature, the sample is indeed present.

Thanks.

[Rafael Chust]

The answers to your questions are mostly answered in this forum under "Assessing an HPLC method for determination of impurities".

[muggle]

thanks =)