Analysis of Glyphosate by TLC

[darwinmani]

Hi all
I`m Mani from India. Currently i am working in Herbicide degradation. Now i need a protocol or article that, how to analyse the Glyphosate, a Herbicide,(Commercial name-Roundup) by Thin Layer Chromatography. Please kindly help me to my mail id (darwinmani@yahoo.co.in)
Thankin you all
regards,
Mani

[chromatographer1]

Try exploring the web site for Camag and see if they have anything you can use.

best wishes,

Rod

[ccyting]

I am not sure you can do TLC on glyphosate. It is a water soluble only compound. If you put Roundup herbide in organic solvent, the glyphosate will be salted out. If you can get any TLC information, please let me know. Thank you and good luck.

[AA]

Young JC, Khan SU & Marriage PB (1977) Fluorescence detection and
determination of glyphosate via its N-nitroso derivative by
thin-layer chromatography. J Agric Food Chem, 25: 918-922.

Ragab MTH (1978) Thin-layer chromatographic detection of glyphosate
herbicide (N-phosphonomethyl glycine) and its aminomethyl phosphonic
acid metabolite. Chemosphere, 2: 143-153.

[michaldousa]

I am sure you can not do TLC on glyphosate. We doing glyphosate by HPLC with post-column derivatization and we have problem get detection limit 1 µg/l. Citation that are undermentioned are suitable for semiquantification only. I have no good experience by all those.

1. Young JC, Khan SU & Marriage PB (1977) Fluorescence detection and
determination of glyphosate via its N-nitroso derivative by
thin-layer chromatography. J Agric Food Chem, 25: 918-922.

2. Ragab MTH (1978) Thin-layer chromatographic detection of glyphosate
herbicide (N-phosphonomethyl glycine) and its aminomethyl phosphonic
acid metabolite. Chemosphere, 2: 143-153.