pseudocyanocobalamins

[chris knutsen]

Does anyone have any HPLC methods for the pseudocyanocobalamins (hydroxycyanocobalamin, etc), especially in the presence of cyanocobalamin? Also, any information about any stability methods for cyanocobalamin?

Thanks for your help in advance.

[Tony_Montanari]

Check out Grace/Alltech for a B12 immuno-affinity column that can be run in conjunction with a switching valve. I got a broshure on it called #522. Perhaps you can use it to selectively remove cyanocobalamine interferences from the hydroxy product.

Grace Davidson Discovery Sciences (they bought Alltech)
www.discoverysciences.com
800-255-8324
ask or look for broshure#522

Best of Luck