Analytical HPLC column comparison

[amirchem]

Hallo

Where can I find a good RP column comparison? I am especially interested in comparison of characteristics of stationery phases from Merck, Agilent and Phenomenex.

Best regards

[bartjoosen]

http://www.pharm.kuleuven.ac.be/pharmch ... imilar.htm

[Uwe Neue]

www.waters.com/selectivitychart

[Mach]

Try this one:

http://www.columnselector.com/

[DR]

Phenomenex's web site features a cross reference guide that will clue you in as to the different carbon loads, surface areas etc. for different columns too. (at least it used to, I haven't been there in a while).

[Rafael Chust]

I would go with Mach and DR hints...

[tom jupille]

The USP has two different column selectivity databases (different ways of measuring) up on their web site. I believe this is a beta-test:
http://www.usp.org/USPNF/columnsDB.html

[syx]

Could you please explain the abbreviations found in the USP website? I could not find the description about them in there…
USP Database: F - Hy - CTF - CFA - TFA - BD
PQRI Database: H - S - A - B - C(2. - C(7.0)

[Uwe Neue]

The webside is still growing, and such things as explanations of the stuff need to be added, as well as more data on the USP data base, and a few other things...

In both cases, the F-factor is a matching factor between the reference column and the closest columns in the data bank.

For the USP data, HY describes the hydrophobicity of the packing, CTF is the tailing factor for a chelator, CFA is the capacity factor of amitriptyline, a basic compound, TFA is the tailing factor of amitriptyline, and BD is the bonding density of the packing.

For the PQRI data base, you have a parameter for the Hydrophobicity of the packing, one for the Steric selectivity, one for hydrogen bond Acidity, one for hydrogen bond Basicity, and C for ion-exChange at pH 2.5 and pH 7.0.