Viscous Heat Generation in UPLC

[Kostas Petritis]

Yury,

I do not understand why you are so negative about it. UPLC has some fundamental advantages and there are just engineering problems that makes it difficult to go to higher pressures. There are groups thought that are working on these problems so I do not find it impossible that some day we might reach the 50000 psi, 1 um particles and robust operation.

Actually the pressures and particles that you mention have already been developed in research laboratories, and put into practice, providing peak capacities not far from the theoretical ones...

[juddc]

Yury -

Some labs need high throughput, others not so much. If you need to put out lots of data fast and your application is consistent with what the machine can do, this machine will do the job very well, saving time and unltimately money.

I'm not God's gift to chromatography, but I've been a practical, "feet on the ground" methods development geek for about the last 15 years and I feel quite comfortable in making that judgement.

With that said, I'm not buying one because I just don't need the throughput and I need broader flexibility in detection (and I already have a raft of different detectors - PDA, RI, flourescence, condictivity, ECD, and soon ELSD that I'd rather not pitch). I'm a one-guy lab and I couldn't prep samples fast enough to keep up with it! I'm only just now going to retire my component system for a 2695....

I've seen the set up and it works quite well. The boost in throughput is IMHO worth the price differential - if you need the throughput.

Chris

[pve]

Hy Thierry,
We are very interested in your colunms for our UPLC's .
It's good to see that a vendor is interested to provide colunms for the UPLC system.
Pierre

[Newman768]

Save your money until you know, how much pressure these columns can handle.
I have seen a lot of small particle columns since the uplc is on the market, but none of them can handle the pressure you normally use with the uplc. Highest I have heard is 600 bar and that is to less to be an alternative to the waters acquity columns.
Next thin is that the acquity columns has a different fitting than other columns, so you get most lieky a dead volume and that can be very nasty to your seperation - making the uplc acting like a bad performing hplc...