Baseline and Tailing

[Yury Zelechonok]

How long is your column?
Is your sample in 85% MeOH as well?
Do you have any other chiral centers in you molecule beside one that shown?

[HW Mueller]

What is your dead time?

Also, unless you have some diastereomeric interactions you would not split enantiomers. For diastereomers the molecule seems a bit small (I don´t discern what it is as yet). Also, I would think that it´s rather unusual to have diastereomers elute together to that conditional extend.

[Basil]

How you prepare your sample solution ?. What pH ?
Do you use the mobil phase to solve your compound ??..

Try to use a solvent weakness than your initial conditions in your mobil phase