HPLC with DMSO as solvent

[b_daniel]

Is it possible to use HPLC to analyze the concentration of small molecules dissolved in DMSO? If not, is there a suitable method?

[james little]

We use DMF and DMSO for dissolving samples for LC-MS analyses.

As long as you keep the injection volume low, 5-10 ul's, causes no serious problems and still gives decent peak shapes.

[amaryl]

Is it possible to use HPLC to analyze the concentration of small molecules dissolved in DMSO? If not, is there a suitable method?

DMSO is used as surfactant to solubilise sample in HPLC. Being a strong solvent should be used in less amount.

Regards,

Amaryl.

[syx]

We have used DMSO (water-free) as diluent for a substance that very sensitive to water. The sample is injected in less than 20 uL and found no significant peak distortion.

[avitan]

syx wrote:

We have used DMSO (water-free) as diluent for a substance that very sensitive to water. The sample is injected in less than 20 uL and found no significant peak distortion.

Was it with Normal-phase or Reversed-phase mode?

[syx]

RP-LC. The mobile phase contains buffer solution. However, the aqueous content of the mobile phase did not degrade the active substance, because the analysis time is not more than 15 minutes.