Merging Chromatographic Peaks

[konkous]

Hello,

I'm currentlly running a method for the quantitative determination of alendronate using a FLD detector. It seems that the peak of alendronate merges with a subsequent peak whose intensity remains constant. In most cases this merging results in a shoulder or extensive tailing of alendronate. Is there a way i can perform a split between these two peaks and are there any criteria for accepting the integrated in such mannner chromatogram?

[Rafael Chust]

Can you give more details on your method? Otherwise, is a bit difficult to give an opinion...

[Rafael Chust]

Looking at your method, you might be suffering either column degradation, since your pH is too high for "normal" silica based columns, or alendroate is partially ionized them generating a splitted peak.

Amines are known to create such problems. I would recommend you to try a Phenomenex Gemini C18 or similar column and increase your pH.