peak area consistently decrease

[jiangds06]

1424616
1423318
1202280
1133670
1137694
1202050

do you know what is the problem, retention time is fixed.

[Gerhard Kratz]

Please, we need more details. My crystal ball is frozen. What sample compound, what column (C18 is not enough), mobile phase etc.?
Could be that your compound is disappearing from the vial, absorption to glass or the stationary phase. Many options.

[uzman]

Actually areas are consistent by pairs. ( 1424616 - 1423318 - 1202280 -1202050 -1133670 -1137694 )
It looks like there is a problem on autosampler .

[jiangds06]

Yes, there two additional samples, which are inserted in between every other two samples.

Actually areas are consistent by pairs. ( 1424616 - 1423318 - 1202280 -1202050 -1133670 -1137694 )
It looks like there is a problem on autosampler .