Glass column chrom & Free Sucrose Quant: Question

[Meerkat]

I have an application that calls for "1 meter x 1/4 in, 2%VOC 60 / 80 supelcoport".

Only have on-hand: glass column with "6 ft. x 1/4in, 3% OVC, 80 / 100".

Application calls for : Helium flow = 40 ml min; Oven 220, hold 30 min; ramp by 25C rate to 350 , hold 25 mins. For assaying free sucrose.

since my on-hand column is different thta what the app calls for, what consequences are there possible?
Does 2% OVC differ from 3% OVC, how so ?
DOes the fact that 1 meter vs. 6 ft have a negative consequence?
Does the 80/100 vs. 60/80 have a negative consequence ?

Thanks in advance for any help team.

[Consumer Products Guy]

My opinion: move the assay to capillary column; get into the 1990s.

We use capillary column for derivatized sucrose determination.

[Meerkat]

My opinion: move the assay to capillary column; get into the 1990s.

We use capillary column for derivatized sucrose determination.

*well, lol @ 'move the assay into the modern era"; however, we have a test method that I'm obligated to follow. Thus, I'll just run some trial injs on this current analytical system with what I have in-hand. And to see that a 60/80 particle size was specified instead of a 100/120 particle size. Yet, I am just a worker-bee so I must follow the method as scripted. I postulate that the effect will be a longer elution time.

[tom jupille]

we have a test method that I'm obligated to follow

Then get the column specified in the test method.

If you change the column, you will have to revalidate the method in any case.

[Meerkat]

we have a test method that I'm obligated to follow

Then get the column specified in the test method.

If you change the column, you will have to revalidate the method in any case.

*yep, you're right, I agree. I'll order the column today, apparantly it's custom-made type--but anywho.

[Meerkat]

we have a test method that I'm obligated to follow

Then get the column specified in the test method.

If you change the column, you will have to revalidate the method in any case.

*well, yeah, but the intent was to run the test sam inj using the column I have available. so the basis of my question was: "does the difference between what I have and what the method says portend any difficulty with the analysis?"

Anywho, it'll take 10 biz days to get the custom made col so maight as well do test injs with what I have in me hand.

[Consumer Products Guy]

Yet, I am just a worker-bee so I must follow the method as scripted.

You can do that, but you're holding back science. We all have a duty to improve upon stuff that is bad or obsolete. Validate the new modification then, don't continue to fall behind.

[KM-USA]

We all have a duty to improve upon stuff that is bad or obsolete.

I'm agreeing with the CPG. This week Verizon cut off support for my analog Nokia 5185i cell phone. So I guess it's time to modernize.

I think they didn't like that my plan was only $10 per month, 30 minutes per month, with no messaging, and of course: no photos.

[Meerkat]

We all have a duty to improve upon stuff that is bad or obsolete.

I'm agreeing with the CPG. This week Verizon cut off support for my analog Nokia 5185i cell phone. So I guess it's time to modernize.

I think they didn't like that my plan was only $10 per month, 30 minutes per month, with no messaging, and of course: no photos.

*Well, by comparing your "telecommunications" limitations to the "analytical limitations" imposed upon me; there could not be a more appropriate metaphor.