Advertisement
Chromatography Forum

avoiding keto enol tautomerism in a compound in HPLC analysi

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Post a reply

avoiding keto enol tautomerism in a compound in HPLC analysi

avatar
saipraveen7
We have been working hard on a compound which exhibits keto-enol tautomerism but we are unable to avoid it. can anyone suggest some tips to avoid this keto enol tautomerism while analysing by HPLC

Re: avoiding keto enol tautomerism in a compound in HPLC ana

avatar
tom jupille
Site Admin
Increase the temperature to speed things up so that you will be looking at the "average" configuration.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374

Re: avoiding keto enol tautomerism in a compound in HPLC ana

avatar
juddc
I don't think you'll ever entirely be rid of it, but pH can play a role too. Assaying for avobenzone works much better at pH ~2.2 than at ~3.0 for this reason.
http://the-ghetto-chromatographer.blogspot.com/
Post a reply
3 posts Page 1 of 1
Return to “Liquid Chromatography”

Who is online

In total there are 13 users online :: 0 registered, 0 hidden and 13 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 13 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry