Hi there !!!
I am using Waters 996 with Empower software which is a good software.
Let me set the example for you.
Suppose i have a actual compound on wavelength is 230 with Mobile phase Meoh/h20 50:50.
Now we run the sample . The peak of interest appears with good resolution.
The question is that if somebody adds any impurity or anything other than my active compound in production process, how come i know that something is added in my sample .
Since i do not get any other response except my compound of interest with my mobile phase mixture at 230 nm.
Naturally the unknown impurity is not necesseraly will elute with my mobile phase combination and still i wish to know any other response within the spectral range of 200-400nm
Please guide me from this point
Thanks
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