Fatty acid analysis

[Jon Woollard]

Hello All,

I have a student who wishes to conduct an analysis of fatty acids. They are Anandamide (arachidonylethanolamide), 2-AG (2-Arachidonylglycerol), and Palmitoylethanolamide. Does anyone have any ideas about the required conditions / mobile phase (I can only do isocratic analysis) or where I should look?

thanks

Jon

[XL]

Jon,

I happen to have some experience analyzing surfactant related compounds. I believe these three are in this catagory. Here is what I would start:

Column: a RP column (C8 or C18), 4.6 x 150 mm, 5 micron should be sufficient

Mobile phase: acetonitrile/D.I. H2O (or NH4OAc buffer for better ELSD response)

Flow rate: 1 mL/min

Detection: ELSD

Here is a link for mor information on surfactant analysis using Acclaim Surfactant column, for your information.

http://www1.dionex.com/en-us/columns_ac ... 25924.html

Good luck,

XL

[Jon Woollard]

Thx XL, that's a good place for us to start

[ym3142]

To me, these are just common neutral organics,