Advertisement

Need urgent help calculating a concentration!!!

Basic questions from students; resources for projects and reports.

8 posts Page 1 of 1
Hi,
i'm facing a very interesting problem and i'd like to hear more oppinions. So, i got a work method wich says the following at the chapter "Standard preparation":
Quote: "Dilute 4ml of 1.25mg/l solution of 'x'(compound is not so important) standard solution in acetonitril to 25ml with diluent.(200ppm)"

So basically it says if i understand correctly, that by diluting 4ml to 25ml, of this 1,25mg/l solution(this solution comes as standard)...i obtain a conc. of 200ppm. According to my calculations the concentration obtained is 0.2ppm
Here's how i calculated:

1,25[mg/l] x 4[ml] / 25[ml] = 0.2[mg/l] = 0.2ppm

Please give me some advice! thank you
Your calculation looks good to me. When discussing this in class you can also point out that "ppm" is a vague term which can mean different things according to how it is expressed - mole/mole, mass/mass, mass/vol etc

Peter
Peter Apps
Your calculation looks good to me. When discussing this in class you can also point out that "ppm" is a vague term which can mean different things according to how it is expressed - mole/mole, mass/mass, mass/vol etc

Peter

Thanks for your reply... i guess in this case it's ppm that stands for mass/volume...no matter how i looked at it, it just didn't add up to 200ppm :)
Your calculation looks good to me. When discussing this in class you can also point out that "ppm" is a vague term which can mean different things according to how it is expressed - mole/mole, mass/mass, mass/vol etc

Peter

Thanks for your reply... i guess in this case it's ppm that stands for mass/volume...no matter how i looked at it, it just didn't add up to 200ppm :)
It is probably supposed to be 200 ppb - doubtless a typo and poor proof reading.

Peter
Peter Apps
Your calculation looks good to me. When discussing this in class you can also point out that "ppm" is a vague term which can mean different things according to how it is expressed - mole/mole, mass/mass, mass/vol etc

Peter

Thanks for your reply... i guess in this case it's ppm that stands for mass/volume...no matter how i looked at it, it just didn't add up to 200ppm :)
It is probably supposed to be 200 ppb - doubtless a typo and poor proof reading.

Peter
Could also be that the standard is 1.25 mg/ml which would make the 200 mg/l correct, still a typo though.
Thanks very much guys...yes, it must be some typo error for sure...now we are trying to find out where the error occured...of course everyone is just like "hm...donno...don't remember" ...very funny situation this. :)
Hi,
i'm facing a very interesting problem and i'd like to hear more oppinions. So, i got a work method wich says the following at the chapter "Standard preparation":
Quote: "Dilute 4ml of 1.25mg/l solution of 'x'(compound is not so important) standard solution in acetonitril to 25ml with diluent.(200ppm)"

So basically it says if i understand correctly, that by diluting 4ml to 25ml, of this 1,25mg/l solution(this solution comes as standard)...i obtain a conc. of 200ppm. According to my calculations the concentration obtained is 0.2ppm
Here's how i calculated:

1,25[mg/l] x 4[ml] / 25[ml] = 0.2[mg/l] = 0.2ppm

Please give me some advice! thank you
200ppb not ppm (type error)
Hi,
i'm facing a very interesting problem and i'd like to hear more oppinions. So, i got a work method wich says the following at the chapter "Standard preparation":
Quote: "Dilute 4ml of 1.25mg/l solution of 'x'(compound is not so important) standard solution in acetonitril to 25ml with diluent.(200ppm)"

So basically it says if i understand correctly, that by diluting 4ml to 25ml, of this 1,25mg/l solution(this solution comes as standard)...i obtain a conc. of 200ppm. According to my calculations the concentration obtained is 0.2ppm
Here's how i calculated:

1,25[mg/l] x 4[ml] / 25[ml] = 0.2[mg/l] = 0.2ppm

Please give me some advice! thank you
200ppb not ppm (type error)
Thanks for your kind reply...greatly appreciated
8 posts Page 1 of 1

Who is online

In total there are 76 users online :: 1 registered, 0 hidden and 75 guests (based on users active over the past 5 minutes)
Most users ever online was 5108 on Wed Nov 05, 2025 8:51 pm

Users browsing this forum: Amazon [Bot] and 75 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry