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- Posts: 148
- Joined: Wed Jul 03, 2013 1:51 am
- Location: Michigan, USA
recently I tried running a reversed phase isocratic separation (8% organic) at higher temperatures to scope the effects on the separation. To my surprise I found that plate number decreased from 12000 to 7000 in response to raising the temperature from 30 - 45C. Mobile phase was water:ACN 92:8 with 0.1% H3PO4. The coulumn was an Agilent Zorbax Eclipse Plus with 5um particles.
This change seemed drastic to me. Why could this be?