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Advances in online viscometry

Posted: Tue Apr 30, 2019 1:40 pm
by CF - David Hills
Online differential viscometers are commonly coupled to gel permeation chromatography (GPC) for molar mass determination via the Mark-Houwink-Sakurada relation, or alternatively the universal calibration technique. However, viscometry’s real strength is realized when coupled with multi-angle light scattering (MALS) to characterize size, shape, branching, and aggregation of polymers and biomolecules, providing deeper insight into macromolecular properties.

New developments in the technology of differential viscometers extend their capabilities to the realm of compact molecules such as proteins. Improved sensor technology in conjunction with a patented thermal balancing system allows for dramatically improved sensitivity and near immunity to pump pulses. Below is a link to an on-demand talk describing a new differential viscometer, incorporating these advances and others, that measures hydrodynamic radius with sensitivity exceeding that of online dynamic light scattering, especially for globular proteins and other compact macromolecules.

To view the presentation click below:

By viewing the presentation you will learn...
    - A high-resolution online bridge viscometer increases sensitivity and dynamic range by more than 3x over previous generation instruments
    - When coupled with MALS, one can measure Rh of large polymers and small biomolecules
    - Patented thermal tuning insures that the bridge is always balanced
    - Lower dispersion bridge preserves peak resolution in multi-detector systems
    - Improved thermal design gives stable baselines for optimal peak detection
    - Innovative pulse suppression insures the measurement is immune to pump pulses