Separating complex mixtures

Questions about Multi-Angle Light Scattering coupled with Size-Exclusion Chromatography or Field-Flow Fractionation

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How can you best separate a complex mixture, which exhibits a broad polydispersity on SEC-MALS, that is not sufficiently separated using two or three columns covering a wide range of molecular weight?
In the case that you cannot find SEC columns which separate your sample, there is another fractionation technique called Field-Flow Fractionation (FFF), which may solve your problem. This technique separates the sample with differential flow velocities; there is no stationary phase, which means that interactions with the stationary phase due to charge or hydrophobic interactions are no longer a concern. FFF fractionates samples across a much larger size range than columns are capable of handling, from 1 nm to 10,000 nm.

[answer provided by Mark Spears, Ph.D., Fujifilm Diosynth Biotechnologies]
Daniel Some, Ph.D.
Principal Scientist
Wyatt Technology Corp.
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