Detecting aggregates

Questions about Multi-Angle Light Scattering coupled with Size-Exclusion Chromatography or Field-Flow Fractionation

2 posts Page 1 of 1
In combining SEC-MALS with other biophysical techniques, why do you see aggregates in light scattering but not in UV?
Like SAXS (small-angle X-ray scattering), light scattering intensity varies with the square of the molecular volume – in other words, it is proportional to the product of molar mass and concentration – whereas UV absorption is only proportional to the concentration. Therefore, light scattering is very sensitive to large aggregates which occur at concentrations too low to detect by UV, and even small amounts will produce a large light-scattering signal.

[answer provided by Kushol Gupta, Ph.D., University of Pennsylvania]
Daniel Some, Ph.D.
Principal Scientist
Wyatt Technology Corp.
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry