How much protein?
Posted: Wed Feb 27, 2019 9:42 am
How much protein do you use in a SEC-MALS experiment?
Typically SEC-MALS requires 50-100 µg, though when necessary as little as 10 µg, assuming the protein elutes well off the column. Experiments aimed at testing self-association will often go to relatively high concentration and use more sample.
[answer provided by Ewa Folta-Stogniew, Ph.D., Director, Keck Biophysical Resource, Yale University School of Medicine]