How much protein?

Questions about Multi-Angle Light Scattering coupled with Size-Exclusion Chromatography or Field-Flow Fractionation

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How much protein do you use in a SEC-MALS experiment?
Typically SEC-MALS requires 50-100 µg, though when necessary as little as 10 µg, assuming the protein elutes well off the column. Experiments aimed at testing self-association will often go to relatively high concentration and use more sample.

[answer provided by Ewa Folta-Stogniew, Ph.D., Director, Keck Biophysical Resource, Yale University School of Medicine]
Daniel Some, Ph.D.
Principal Scientist
Wyatt Technology Corp.
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