Measuring hydrodynamic radius of DNA-protein complex

Questions about Multi-Angle Light Scattering coupled with Size-Exclusion Chromatography or Field-Flow Fractionation

2 posts Page 1 of 1
Can we measure the hydrodynamic radius of a DNA-Protein complex in a SEC-MALS analysis?
Yes, the hydrodynamic radius of the DNA-protein complex may be measured simultaneously with SEC-MALS using online dynamic light scattering (DLS). An internal DLS detector may be added to a MALS detectors in order to measure molar mass and size simultaneously in the same flow cell and measurement volume. Hydrodynamic radius may also be measured by viscometry if an inline differential viscometer is plumbed in line with the MALS and concentration detectors.

[answer provided by Sophia Kenrick, Ph.D., Wyatt Technology]
Daniel Some, Ph.D.
Principal Scientist
Wyatt Technology Corp.
Image
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry