Benefits of SEC-MALS

Questions about Multi-Angle Light Scattering coupled with Size-Exclusion Chromatography or Field-Flow Fractionation

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What, in your opinion, is the primary benefit of SEC-MALS?
The real benefit is the ability to determine, in solution, accurate molecular weights for various macromolecules, including proteins, protein-protein complexes, protein-nucleic acid complexes, PEGylated proteins, membrane proteins that are isolated as complexes with detergents and lipids etc. regardless of the shape of the assembly. SEC-MALS can characterize oligomers that are not amenable to analysis by standard SEC or native PAGE because of the nonglobular shape, interactions with the column media, modifications that affect shape, etc. Another important benefit is the dynamic range of an analytical SEC-MALS system that allows measurements over four orders of magnitude in concentration and molar mass.

[answer provided by Ewa Folta-Stogniew, Ph.D., Director, Keck Biophysical Resource, Yale University School of Medicine]
Daniel Some, Ph.D.
Principal Scientist
Wyatt Technology Corp.
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